Combining the best of two- and four-photon microscopy

Our probes merge the advantages of two-photon microscopy (low excitation energies, standard lasers around 800 nm, and optimal tissue penetration) with the high spatial resolution of four-photon microscopy (150 nm or less, being a substantial improvement compared to one- or two-photon excitation schemes using similar hardware) implying a true paradigm shift in multiphoton microscopy. By leveraging our probes, researchers can achieve four-photon-like resolution while maintaining compatibility with existing two-photon equipment, offering a unique value in a cost-effective way to upgrade without major investments.